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Une fois sorti du moule, le substrat PUMA a été fenty puma collé de manière conforme à une autre lamelle de verre revêtue de PUMA (durci) avec une légère pression mécanique. Cette liaison conforme a été convertie en liaison permanente en plaçant la puce PUMA sous la source d'inondation UV pendant 10 minutes supplémentaires (A) Présentation du matériau pendant le moulage de la puce PUMA. 1: moule PDMS; 2: résine PUMA; 3: Poteaux PTFE; 4: feuille de polypropylène; 5: cellophane (ou Aclar). (B) Deux méthodes d'interfaçage de la puce PUMA avec des tubes externes: 1: puce PUMA; 2: connecteur coudé; 3: tube en polyuréthane; 4: résine supplémentaire pour colmater la fuite (Dymax 186M); 5: tube PTFE; 6: thermorétractable polyoléfine; 7: bague de retenue.
La hauteur de la caractéristique était de ~ 40 ¼m et le rapport d'aspect des colonnes verticales était de ~ 3,5. Un coude à 180 ° a été incorporé dans la conception pour puma fenty aider au dépannage s'il y avait des problèmes directionnels dans le processus de réplication ou de démoulage. Comme le montre la figure 2A, les colonnes produites dans PUMA avaient un profil vertical net sans aucune trace d'inclinaison.Nous avons constaté que pour les caractéristiques à faible rapport d'aspect (H / W <�1), puma suede femme la résine PUMA durcie pouvait être libérée du PDMS mouler soit (1) en décollant légèrement le moule de la résine durcie, soit (2) en calant un scalpel entre la résine et le moule pour soulever doucement la résine.
Deux types de défauts étaient évidents: (1) les murs ondulés et (2) les espacements verticaux irréguliers des colonnes. L'ondulation de la paroi est généralement attribuable à un nettoyage incorrect du moule PDMS entre les cycles de réplication, ce qui augmente l'adhérence entre le moule et la résine. Par conséquent, il n'est pas possible de se séparer sans un certain mouvement horizontal pour contrer la puma basket force d'adhérence. Les moules PDMS frais et inutilisés ne présentaient pas ce problème. La sonication rigoureuse des moules PDMS dans l'isopropanol et l'eau entre les répétitions a considérablement réduit les incidents de murs incurvés défectueux.
L'espacement entre les colonnes verticales était périodique comme prévu (pas de taches sombres irrégulières) .Nous avons également essayé plusieurs processus de modification de surface sur PDMS pour améliorer le processus de démoulage, y compris l'oxydation plasma, la silanisation avec (tridécafluoro-1,1,2,2-tétrahydrooctyle ) le tri-chlorosilane et le revêtement de surfactants tels que le n-dodécyl-bêta-D-maltoside (DDM), 19 Gransurf 71 et Gransurf 77. Ces techniques de modification de surface n'ont pas amélioré le processus de démoulage; dans le cas de la silanisation, la surface du PDMS silanisé est devenue trop hydrophobe pour que la résine PUMA non durcie se mouille correctement.
Nous avons évité l'empreinte de la crête en tapissant le sac sous vide avec un chiffon non pelucheux. La puce a été retirée du sac en plastique une fois que> 80% de la zone a été scellée de manière conforme. Les rayons UV et la chaleur intenses de la source ont provoqué le ramollissement du substrat PUMA et le joint conforme est devenu une liaison permanente pendant le processus de refusion. La refusion n'a pas puma rihanna entraîné de distorsion des microstructures tant qu'aucune pression n'était appliquée au-dessus de la puce alors qu'elle était encore molle.
Une fois la puce refroidie, le joint permanent était capable de résister à un débit élevé et soutenu (> 1 ml / min) à haute pression (20 à 40 psi); nous avons régulièrement observé que la lamelle couvre-objet du microscope (n ° 2), qui constituait la surface inférieure de la puce, s'est fracturée avant la rupture du joint permanent. Cette méthode de collage est notre méthode de choix; cependant, d'autres techniques de liaison peuvent également être utilisées, que nous décrivons brièvement ci-dessous.